RBM15 Knockout cell line (Hep G2)

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RBM15 Knockout cell line (Hep G2) Order

RBM15 Knockout cell line (Hep G2)
Catalog#
YKO-HT4247
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RBM15 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RBM15 Knockout cell line (Hep G2)
Species
Gene Name
RBM15
Gene ID
64783
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RBM15 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RBM15 gene knockout RBM15 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RBM15 Knockout cell line (Hep G2)
RBM15 Knockout cell line (Hep G2)
Catalog#
YKO-HT4247
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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